FURTHER BIOCHEMICAL AND MORPHOLOGICAL STUDIES OF GRANULE FRACTIONS FROM RABBIT HETEROPHIL LEUKOCYTES
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چکیده
منابع مشابه
Further Biochemical and Morphological Studies of Granule Fractions from Rabbit Heterophil Leukocytes
Fractionation of rabbit heterophil leukocyte homogenates by isopycnic centrifugation as well as by zonal sedimentation has helped to characterize further the particulate components of these cells. Four classes have been identified: (A) Large (0.5-0.8 microm) and dense (1.26) azurophil or primary granules, containing all the myeloperoxidase, one-third of the lysozyme, and a major proportion of t...
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When rabbit peritoneal exudates (97% polymorphonuclear [PMN] leukocytes, 2% mononuclear cells) were fractionated by zonal sedimentation or isopycnic centrifugation, four fractions (A, B, C, and D) were obtained, as reported earlier. "A" consisted largely of PMN azurophil granules, "B" of PMN specific granules, and "D" of membranous elements. The source of the more heterogeneous "C" fraction (co...
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Postnuclear supernates from homogenates of essentially pure rabbit heterophil leukocytes were fractionated by means of zonal differential centrifugation through a discontinuous sucrose gradient at various speeds. Three distinct groups of granules were characterized biochemically and morphologically. They were, in order of decreasing sedimentation coefficient: (a) Large, relatively dense granule...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1970
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.45.3.586